Sticky ends from different CsiI sites may not be compatible. Sticky ends from different DraIII sites may not be compatible. SmaI can be used at 37☌ for brief incubations.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.īclI is typically used at 50-55☌, but is 50% active at 37☌. Sticky ends from different AccI sites may not be compatible.Įfficient cleavage requires at least two copies of the SacII recognition sequence.Ĭleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. PaeR7I does not recognize the sequence CTCTCGAG.Įfficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence. Prolonged incubation with NdeI may lead to removal of additional nucleotides.īsrGI is typically used at 37☌, but is even more active at 60☌. Sticky ends from different SfiI sites may not be compatible.
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